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monoclonal mouse anti flag tag antibody  (Novus Biologicals)


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    Novus Biologicals monoclonal mouse anti flag tag antibody
    Monoclonal Mouse Anti Flag Tag Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flagellin/Flagellin+Antibody+(06)+%5BDyLight+350%5D/bio_rxiv__64898__2026__03__27__714707-459-0-32
    Average 94 stars, based on 1 article reviews
    monoclonal mouse anti flag tag antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Incubation:

    Article Title: Diabetic pregnancy activates the innate immune response through TLR5 or TLR1/2 on neonatal monocyte.
    Article Snippet: .. Cord blood monocytes were transferred to 96-well plates (105 cells/well) and left untreated (controls) or incubated with lipopolysaccharide (LPS; TLR4 ligand; 10, 100, and 1000 ng/ml; Escherichia coli serotype O111 B4, Sigma, St. Louis, MO, USA), flagellin (TLR5 ligand; 1, 10, and 100 ng/ml; catalog no. IMG-2205, Imgenex, San Diego, CA, USA), zymosan (TLR2 and TLR6 heterodimer ligand; 0.1, 1, and 10 g/ml; catalog no. IMG-2212, Imgenex), Pam3CSK4 (TLR1 and TLR2 heterodimer ligand; 10, 100, and 1000 ng/ml; catalog no. IMG-2201, Imgenex), and macrophage-activating lipopeptide (MALP; TLR2 and TLR6 heterodimer ligand; 10, 100, and 1000 ng/ml; catalog no. IMG-2206, Imgenex) for 12 h at 37 ◦C in humidified air containing 5% CO2. ..

    Article Title: Molecular cloning and functional studies on magang goose toll-like receptor 5.
    Article Snippet: Disease outbreaks heavily impact the economic viability of animal industries.. Little is known about the mechanisms of immune system-related diseases in geese.. Toll-like receptors (TLRs) play a major role in the antiinflammatory immunity process in most animal species, but they have not been studied in the Magang goose.

    Control:

    Article Title: Molecular cloning and functional studies on magang goose toll-like receptor 5.
    Article Snippet: Disease outbreaks heavily impact the economic viability of animal industries.. Little is known about the mechanisms of immune system-related diseases in geese.. Toll-like receptors (TLRs) play a major role in the antiinflammatory immunity process in most animal species, but they have not been studied in the Magang goose.

    Concentration Assay:

    Article Title: The expression of Toll-like receptor 5 in preterm histologic chorioamnionitis
    Article Snippet: The intact tissues pieces were cultured in DMEM/F12 (11039-021; Thermo Fisher Scientific) containing 10% fetal calf serum (FCS) (Biological Industries, Haemek, Israel), 100 U/ml penicillin G (Meiji Seika Pharma Co., Ltd., Tokyo, Japan), 100 μg/ml streptomycin (Meiji Seika Pharma Co., Ltd.) and 2.5 μg/ml amphotericin B (Sigma Aldrich, St. Louis, MO) at 37°C under a 5% CO 2 atmosphere for 8 h. The tissues were incubated in DMEM/F12 at 37°C under a 5% CO 2 atmosphere, with or without the treatment of agents after 1 h of starvation. .. The concentration of each ligand was determined as a previous report: 100 ng/ml of Pam3Cys-Ser-(Lys)4 (Novus Biologicals, Littleton, CO); 50 μg/ml of poly(I:C) (Novus Biologicals); 1 μg/ml of LPS (Sigma-Aldrich Japan, Tokyo, Japan); 100 ng/ml of flagellin (Novus Biologicals); and 100 ng/ml of MALP2 (Novus Biologicals). ( ) TNF-α at a concentration of 10 ng/ml (Novus Biologicals) and 10 ng/ml of IL-1β (Novus Biologicals) were also used. ..

    Injection:

    Article Title: TLR1/2 ligand enhances antitumor efficacy of CTLA-4 blockade by increasing intratumoral Treg depletion
    Article Snippet: .. Pam3CSK4, LPS, and HKST were purchased from Invivogen, whereas Flagellin was purchased from Novus Biologicals and injected intratumorally. .. Ribopure RNA purification kit was purchased from Thermo Fisher Scientific.

    Purification:

    Article Title: Toll-Like Receptor 1/2 and 5 Ligands Enhance the Expression of Cyclin D1 and D3 and Induce Proliferation in Mantle Cell Lymphoma
    Article Snippet: .. Cells were activated with soluble CD40L (Alexis, 1 μg/ml), 1 μg/ml of the corresponding enhancer (Enzo Life Sciences), 10 ng/ml of IL-4 (R&D Systems), and 2 μg/ml F(ab’) 2 fragment Goat Anti-Human IgM (Jackson ImmunoResearch), and treated for 72 or 96 hours with 30 ng/ml Pam 3 CSK 4 (TLR1/2 ligand; Imgenex), or 75 ng/ml purified flagellin (TLR5 ligand; Imgenex). .. Mino, SP53, and Jeko-1 cell lines were contributed by Dr. Raymond Lai.

    other:

    Article Title: Loss of myeloid-specific protein phosphatase 2A enhances lung injury and fibrosis and results in IL-10-dependent sensitization of epithelial cell apoptosis
    Article Snippet: CpG ODN 1668 (1 μM, cat. no. IMG-2209), Poly I:C (10 μg/ml, cat. no. IMG-2203), and Pam3CSK4 (1 μg/ml, cat. no. IMG-2201) were purchased from Imgenex.



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    TMH does not affect other inflammasome or inflammatory pathways. (A,B) LPS-primed J774A.1 cells were treated with TMH for 2 h, followed by stimulation with either dsDNA (1 μg/mL) (A) or <t>flagellin</t> (B) transfected using Lipofectamine 2000 for 3 h. (C) Following TMH treatment (2 h), nuclear and cytosolic fractions of LPS-primed J774A.1 cells were separated and assessed for NF-κB p65 translocation by immunoblotting. (D) HEK293FT cells transfected with the pGL4.32 luciferase reporter vector were treated with TNF-α (20 ng/mL) for 5 h, with or without TMH pre-treatment (2 h). Luciferase activity was measured using the Bright-Glo™ luciferase assay system (Promega). Data are presented as the mean ± SD (n = 3). Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test n.s., not significant.
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    monoclonal mouse anti flag tag antibody  (Novus Biologicals)
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    TMH does not affect other inflammasome or inflammatory pathways. (A,B) LPS-primed J774A.1 cells were treated with TMH for 2 h, followed by stimulation with either dsDNA (1 μg/mL) (A) or <t>flagellin</t> (B) transfected using Lipofectamine 2000 for 3 h. (C) Following TMH treatment (2 h), nuclear and cytosolic fractions of LPS-primed J774A.1 cells were separated and assessed for NF-κB p65 translocation by immunoblotting. (D) HEK293FT cells transfected with the pGL4.32 luciferase reporter vector were treated with TNF-α (20 ng/mL) for 5 h, with or without TMH pre-treatment (2 h). Luciferase activity was measured using the Bright-Glo™ luciferase assay system (Promega). Data are presented as the mean ± SD (n = 3). Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test n.s., not significant.
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    flag  (Novus Biologicals)
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    Image Search Results


    TMH does not affect other inflammasome or inflammatory pathways. (A,B) LPS-primed J774A.1 cells were treated with TMH for 2 h, followed by stimulation with either dsDNA (1 μg/mL) (A) or flagellin (B) transfected using Lipofectamine 2000 for 3 h. (C) Following TMH treatment (2 h), nuclear and cytosolic fractions of LPS-primed J774A.1 cells were separated and assessed for NF-κB p65 translocation by immunoblotting. (D) HEK293FT cells transfected with the pGL4.32 luciferase reporter vector were treated with TNF-α (20 ng/mL) for 5 h, with or without TMH pre-treatment (2 h). Luciferase activity was measured using the Bright-Glo™ luciferase assay system (Promega). Data are presented as the mean ± SD (n = 3). Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test n.s., not significant.

    Journal: Frontiers in Pharmacology

    Article Title: Thymelaea hirsuta (L.) Endl . extract attenuates NLRP3 inflammasome activation via modulation of ATPase activity

    doi: 10.3389/fphar.2026.1781860

    Figure Lengend Snippet: TMH does not affect other inflammasome or inflammatory pathways. (A,B) LPS-primed J774A.1 cells were treated with TMH for 2 h, followed by stimulation with either dsDNA (1 μg/mL) (A) or flagellin (B) transfected using Lipofectamine 2000 for 3 h. (C) Following TMH treatment (2 h), nuclear and cytosolic fractions of LPS-primed J774A.1 cells were separated and assessed for NF-κB p65 translocation by immunoblotting. (D) HEK293FT cells transfected with the pGL4.32 luciferase reporter vector were treated with TNF-α (20 ng/mL) for 5 h, with or without TMH pre-treatment (2 h). Luciferase activity was measured using the Bright-Glo™ luciferase assay system (Promega). Data are presented as the mean ± SD (n = 3). Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test n.s., not significant.

    Article Snippet: For inflammasome stimulation, ultrapure LPS (tlrl-ppglps), nigericin (tlrl-nig), adenosine triphosphate (ATP; tlrl-atpl), and flagellin (FLA-ST; tlrl-epstfla) were obtained from InvivoGen (United States).

    Techniques: Transfection, Translocation Assay, Western Blot, Luciferase, Plasmid Preparation, Activity Assay